芒果苷元在大鼠血浆中的含量测定及药动学研究

岳静静, 索绪斌, 游杨杨, 张涵

中国药学杂志 ›› 2017, Vol. 52 ›› Issue (10) : 857-860.

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中国药学杂志 ›› 2017, Vol. 52 ›› Issue (10) : 857-860. DOI: 10.11669/cpj.2017.10.011
论著

芒果苷元在大鼠血浆中的含量测定及药动学研究

  • 岳静静1, 索绪斌1*, 游杨杨1, 张涵2
作者信息 +

Determination of Norathyriol in Rat Plasma and Its Pharmacokinetics Study

  • YUE Jing-jing1, SUO Xu-bin1*, YOU Yang-yang1, ZHANG Han2
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摘要

目的 建立测定大鼠血浆中芒果苷元含量的HPLC,探讨其在大鼠胃肠道给药后的药动学过程。方法 选择对硝基苯酚为内标物质,大鼠血浆样品用乙酸乙酯-甲酸(10:1)进行萃取,用HPLC进行分析。色谱条件:色谱柱:Platisil 5 μm ODS(4.6 mm×250 mm);流动相:乙腈-水-甲酸(40:60:0.5,pH 2.74);柱温:室温;流速:0.8 mL·min-1;检测波长:312 nm。单剂量灌胃给药400 mg·kg -1后,测定不同时间点大鼠血浆中芒果苷元的浓度。结果 芒果苷元在0.54~ 162 μg·mL-1血浆浓度范围内呈现良好线性关系(r=0.998)。日内和日间精密度RSD均小于7.5%。利用软件Winnonlin 6.1计算其主要药动学参数为:tmax为0.5 h;t1/2为(3.46±0.903)h;ρmax为(26.9±3.17)μg·mL-1;AUC0-t为(52.4±12.0) μg·h·mL-1结论 本试验所建立的HPLC测定大鼠血浆中芒果苷元含量的方法简单可行,专属性强,可用于大鼠血浆中芒果苷元的药动学分析。

Abstract

OBJECTIVE To establish a method for the determination of norathyriol in rat plasma by HPLC and to study its characteristics of pharmacokinetics. METHODS Ethyl formic acid(10:1)was used to precipitate protein in the plasma samples after the addition of internal standard, and then the concentration was analyzed by HPLC. All of the separations were carried out on a Platisil ODS(4.6 mm×250 mm,5 μm) at room temperature. The mobile phase was consisted of formic acid(40:60:0.5, pH 2.74), and was pumped at flow rate of 0.8 mL·min-1. The UV detection wavelength was set at 312 nm. The rats were given norathriol by intragastric administration with a dosage of 400 mg·kg-1. The concentration of norathriol in plasma at different time points was determined. RESULTS A good linear relationship was obtained in the concentration range of 0.54-162 μg·mL-1(r=0.998). The intra-day and inter-day RSD were less than 7.5% . The main pharmacokinetic parameters measured by Winnonlin 6.1 were showed as follows:tmax,t1/2, ρmax and AUC0-t were 0.5 h,(3.46±0.903) h,(26.9±3.17)μg·mL-1,(52.4±12.0) μg·h·mL-1, respectively. CONCLUSION The HPLC method established is rapid and specific, and can be successfully applied in basic pharmacokinetic study in rat plasma.

关键词

芒果苷元 / 大鼠 / 血浆 / 药动学 / 高效液相色谱法

Key words

norathyriol / rat / plasma / pharmacokinetics / HPLC

引用本文

导出引用
岳静静, 索绪斌, 游杨杨, 张涵. 芒果苷元在大鼠血浆中的含量测定及药动学研究[J]. 中国药学杂志, 2017, 52(10): 857-860 https://doi.org/10.11669/cpj.2017.10.011
YUE Jing-jing, SUO Xu-bin, YOU Yang-yang, ZHANG Han. Determination of Norathyriol in Rat Plasma and Its Pharmacokinetics Study[J]. Chinese Pharmaceutical Journal, 2017, 52(10): 857-860 https://doi.org/10.11669/cpj.2017.10.011
中图分类号: R969.1   

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基金

国家自然科学基金资助项目(81303288);广东省科技创新专项资金(2014A0101112);广东省教育厅创新强校特色创新项目(粤教科函[2015]3号);广州科技型中小企业创新基金(2017010160063)
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